Custom DNA Oligonucleotide Synthesis

Millions of oligonucleotides are synthesized every year for use in laboratories around the world. For most applications, very small amounts of DNA oligonucleotides are required, and the synthesis is primarily performed on a scale of 40 nmol or less, which provides sufficient quantities for most biochemical and biological experiments. 10 µmol or more of DNA can be prepared for biophysical studies (NMR and X-ray crystallography) and solid-phase methods may have been developed to synthesize several kilograms of oligonucleotides as drug molecules^[2].

Methods of DNA Oligo Synthesis

Subsequent improvements to oligonucleotide synthesis chemistries and techniques in the early 1980s led to the development of solid-phase phosphoramidite chemistries, whose robustness and fidelity allowed for automated methods to be developed to enable scalable oligonucleotide synthesis that is used to this day for the commercial synthesis of oligonucleotides^[2]. Single-stranded DNA fragments of less than 200 nt are mainly produced by phosphoramidite chemistry methods, the direct chemical synthesis methods that use either traditional column-based synthesizers or microarray-based synthesizers^[1].

Phosphoramidite-based oligonucleotide synthesis^[2]

Our Custom DNA Synthesis Service

Our custom DNA synthesis service can be tailored to customers' needs. We have fully automatic synthesizers of various specifications and advanced purification equipment. We provide general DNA synthesis products and modified DNA synthesis products. The wide range of DNA oligonucleotide modifications includes DNA backbone modification, modified bases, and fluorescent dye labeling. There are three levels of DNA synthesis products: Standard purification, PAGE purification, and HPLC purification. You can choose the appropriate level of products according to your experimental needs.

We prepare our DNA oligonucleotides with strict quality control and fast run cycles. They can be used for a variety of assays, including PCR, cloning, sequencing, and genetic testing. Every DNA product is quality checked using MALDI-TOF and Electrospray Ionization (ESI) Mass Spec and OD by UV spectroscopy.

Modifications

Guide of Purification Methods

1. Standard Purification
   The product purity can reach 80-90% in general, suitable for use as PCR primers, DNA sequencing primers, various probes, etc. However, this level is effective for DNA products below 39mer. When the sequence is longer, the purity of the product cannot be guaranteed, so it is recommended to use PAGE purified products.
2. PAGE Purification
   The purity of the product can reach up to 90 ~ 95%, suitable for use as RT-PCR primers, various probes, or for PCR cloning and sequencing, site-directed mutagenesis, etc.
3. HPLC Purification
   The purity of the product can be up to 95%. Due to the very high purity, the DNA products purified by this method can be applied to various genetic engineering experiments, in particular, PCR cloning, site-directed mutagenesis, artificial synthesis of genes, etc.

Competitive Advantages:

• Access hundreds of chemical modifications at a competitive price
• Comprehensive modifications and labeling
• Flexible synthesis scales
• Bulk orders are available upon request.
• Stringent quality control
• High-quality and cost-effective
• Both unmodified and modified DNA oligos are available.
• Comprehensive backbones
• Fast turnaround: 5-10 business days

Delivery:

• Synthetic DNA in tubes
• COA
• Test report (MS & HPLC)

References

1. Randall A. Hughes, Andrew D. Ellington Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology. Cold Spring Harb Perspect Biol. 2017 Jan; 9(1): a023812.
2. Min Hao, Jianjun Qiao, Hao Qi. Current and Emerging Methods for the Synthesis of Single-Stranded DNA. Genes. 2020, 11(2), 116

*For Research Use Only. Not for use in diagnostic procedures.

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