Design and Chemical Modification of miRNA and Anti-miRNA Oligo
MicroRNA (miRNA) is single-stranded RNA molecules with endogenous length of 18-22nt, which plays an important role in controlling gene expression after transcription. From embryonic development to cell apoptosis and tumor growth, miRNA is one of the most important factors in the process. The complex and detailed regulatory mechanisms of miRNA make the entire signaling pathway a promising therapeutic drug (usually in the form of miRNA analogs) or a therapeutic target (usually in the form of anti-miRNAs) in many pathological processes. Creative Biolabs can improve RNA molecular delivery technology to achieve miRNA-based disease treatment protocols. Common delivery systems include lipid nanoparticles, such as neutral fat emulsions and dendritic macromolecular complexes that target partially connected molecules.
Creative Biolabs provides customers with miRNA and anti-miRNA oligo services, providing more choices in the direction of design and chemical modification of miRNA and anti-miRNA oligo.
Our miRNA and Anti-miRNA Oligo service:
Creative Biolabs can provide customers with miRNAs of different forms, different lengths and designed by customers themselves, which is convenient for customers to choose. Creative Biolabs can provide multiple purification methods and comprehensive quality control reports.
- Synthesize customized miRNA and anti-miRNA oligo ODN. The miRNA is difficult to be separated due to their low content. Once isolated, these 18-24nt RNAs which need to be purified, coupled with 5' and 3' terminals and sequenced by RT-PCR are cloned into the vector. The computational analysis of genome sequences is often used as a practical tool to make up for the shortcomings of cloning. The new miRNAs identified by this approach must be modified by means of computational analysis.
- Clone sequences that you are interested in using miRNA technology.
- Provide miRNA and anti-miRNA oligo construction services. We can assist customers to design gene sequences that can be cloned into any miRNA cloning vectors.
- The purified miRNA expression plasmid can be directly used for transfection, quantitative analysis in northern blotting and dot blotting, RNase protection assay, primer amplification analysis, infection test and quantitative PCR.
- Antisense technique. Creative Biolabs provides antisense techniques that specifically inhibit miRNA activity. Antisense oligonucleotide technology mainly includes antisense oligonucleotide technology and antisense RNA technology. At present, antisense oligonucleotide technology is widely used for miRNA. Antisense oligonucleotide technology refers to the design of synthetic antisense oligonucleotides that are complementary to miRNA, which is anti-miRNA oligonucleotide (AMO). When AMO is introduced into cells or in vivo, it is specifically bound with miRNA to form AMO-miRNA hybridization, preventing miRNA from binding to target mRNA and activating RNaseH to degrade miRNA. Common ways of chemical modification include phosphoro-thioate backbone, 2'-O-methyl, 2'-O-methoxyethyl, 2'- flouro, locked nucleic acid and peptide nucleic acid.
Advantages of miRNA and anti-miRNA oligo service:
- High efficiency: The designed product can be multiplied and regenerated. After use, there is no need to recombine.
- Ready to use: It can be used directly without subcloning and other operations.
- Flexible service: Creative Biolabs provides paid commercial carrier for customers. Creative Biolabs's unique tools will help customers solve miRNA and anti-miRNA oligo design problems for specific molecular structures.
References
- Lima JF, et al. Anti-miRNA oligonucleotides: A comprehensive guide for design. RNA Biol. 2018, 15(3):338-352.
- Lu TX, et al. MicroRNA. J Allergy Clin Immunol. 2018, 141(4):1202-1207.
*For Research Use Only. Not for use in diagnostic procedures.
