Evaluation of Specific Receptor Affinity
The receptor is the biological macromolecule that binds to hormones, neurotransmitters, drugs or intracellular signaling molecules and can cause changes in cell function. The ability of a receptor to bind ligands (drugs, transmitters, hormones and autologous active substances) is called affinity. Affinity information can be obtained by dynamics analysis of ligand-receptor binding reactions. The receptor has a strong affinity for its ligand, and the stronger the affinity, the more likely the receptor is to be occupied. Receptor dynamics studies the quantitative relationships and laws of ligands binding to receptors and causing biological effects. In receptor binding experiments, certain ligands are commonly used to detect receptor binding. The evaluation methods for specific receptor affinity include functional experimental analysis, radio-ligand receptor binding assay, affinity chromatography, interface optics method, magnetic resonance imaging technique, capillary electrophoresis assay and liquid scintillating counting.
Creative Biolabs provides advanced open nucleic acid drug technology platform and overall solution, providing customers with comprehensive and highly customized specific receptor affinity evaluation services and saving valuable time and cost for partners.
We provide but are not limited to:
- Radio-ligand receptor binding assay. The interaction between ligand and receptor binding sites complies with the law of mass action. By using different concentrations of radioactive ligands to bind a certain amount of receptors, the content of receptors can be derived by mathematical mapping, and the dissociation constant can be calculated as an index of affinity between receptors and ligands.
- To study specific receptors with radioactive ligands, Creative Biolabs takes a certain approach to eliminate the influence of the binding of non-specific receptors. The general and non-specific bonded tubes are prepared respectively. The difference between the two counts is the specific binding amount.
- The basic requirements of radioactive ligands are high specific activity, high affinity, high specificity and good stability.
- If the affinity between the ligand and the receptor is low, Creative Biolabs will take more steps to deal with the ligands to achieve basic saturation before subsequent evaluation. Lack of timely and appropriate ligand treatment will not only increase the non-specific combination, but also greatly increase the cost.
- The radionuclides commonly used in receptor radioanalysis are 3H and 125I.
- Affinity chromatography. Affinity chromatography is a chromatography method in which one of the two substances with a highly specific affinity is used as a stationary phase. Different degree of affinity with fixed phase can be used to separate the components from impurities.
- Liquid scintillating counting. Liquid scintillating counting is a kind of radioactive measurement technology with the help of scintillating liquid as the medium of ray energy transmission. The technical feature is to completely dissolve or uniformly disperse the sample in the liquid scintillation, or to suspend the sample in the scintillation liquid, or to adsorb the sample on the solid support and immerse it in the scintillation liquid, so as to be in close contact with the scintillation liquid. The advantage is that the self-absorption of rays in the sample is very low, and there is no such problem as the absorption of detector wall, window and air.
References
- Naklua W, et al. Dopaminergic receptor-ligand binding assays based on molecularly imprinted polymers on quartz crystal microbalance sensors. Biosens Bioelectron. 2016, 81:117-124.
- Temple S. Liquid scintillation counting: how has it advanced over the years and what does the future hold? Bioanalysis. 2015, 7(5):503-505
*For Research Use Only. Not for use in diagnostic procedures.
