Gene Manipulation Service
Creative Biolabs can combine genetic material (homologous or heterologous, prokaryotic or eukaryotic, natural or synthetic DNA) from a variety of sources in vitro with viral and bacterial plasmids in the carrier system using biochemical methods to conduct gene manipulation. As a world leading pharmaceutical research and development service company, Creative Biolabs has a world-class team with rich experience in gene manipulation. Creative Biolabs has an advanced gene manipulation technology platform, which can provide customized service to suit customers' specifications and support your target research. Creative Biolabs offers a one-stop service to accompany your entire research.
We provide but are not limited to:
- Separation and purification of nucleic acids
Creative Biolabs can assist customers to analyze the structure and function of target nucleic acid drugs, and use the physical and chemical properties of nucleic acids for gene manipulation. Creative Biolabs can analyze the basic process of genetic information transfer and apply it to the gene operation process required by customers. Creative Biolabs summarizes the principle and basic steps of the separation and purification of nucleic acid drug, and converts them to clients. According to customer requirements, we can do total DNA extraction and detection, as well as plasmid DNA extraction and detection. Creative Biolabs can provide service of RNA extraction and detection.
- Cloning of the target gene
Creative Biolabs can assist customers to program PCR reaction and provide PCR amplification equipment for series of analysis. Creative Biolabs provides PCR primer design services to deal with problems in the PCR process. Creative Biolabs can provide enzymatic reaction operation service according to customer demand.
- In vitro DNA recombination
Creative Biolabs can assist customers to connect DNA fragments in vitro. Creative Biolabs provides recombinant DNA transformation and recombinant DNA screening and identification services. The service of in vitro DNA recombination can realize the construction and transformation, as well as screening and identification of recombinant DNA, helping clients to speed up the research and development of new nucleic acid drugs.
- Expression and identification of target genes
- SDS-PAGE. Depending on the molecular weight of the protein, Creative Biolabs can provide different concentrations of the gel, thus regulating the pore size of the gel and ultimately enabling the separation of proteins with different molecular weights. SDS-PAGE has the advantages of high cost performance, low sample consumption, relatively simple operation and good repeatability. Due to its high resolution, this service is superior to the common chromatography, hypercentrifugation and agarose gel electrophoresis in many cases. This technology service can not only isolate proteins, but also nucleic acids, and is widely used in the separation, identification, detection, qualitative and quantitative analysis of biological macromolecules.
- Western Blot. In this service, the processed cell and biological tissue samples by gel electrophoresis are stained with specific antibodies. By analyzing the location and depth of staining, the western blot service can obtain information about the expression of a specific protein in the analyzed cell or tissue. Because of the high resolution of SDS-PAGE and the high specificity and sensitivity of solid phase immunoassay, western blot has become a routine technique for protein analysis. It is often used to identify proteins and perform qualitative and semi-quantitative analysis of proteins.
Creative Biolabs can provide service for induction and expression of target genes. By using SDS-PAGE electrophoresis and Western Blot hybridization, Creative Biolabs can realize the transmission and translation of genetic information, the regulation of gene expression, the expression and identification of target genes.
References
- Jordan K, et al. Gene Manipulation[M]// Listeria monocytogenes in the Food Processing Environment. Springer International Publishing, 2015.
- Baffa R, et al. Analyzing the FHIT Gene by RT-PCR, Western Blotting, and Immunohistochemistry[J]. Methods in Molecular Medicine, 2001, 53:81.
*For Research Use Only. Not for use in diagnostic procedures.
