Hepatotoxicity Analysis Service
It is important to evaluate drug toxicity in the early stage of drug screening, because the toxic injury of all kinds of tissues and organs is a common cause of failure in drug development. Early detection of potential toxicity can lead to significant savings in development cost and improve drug conversion rates. Drug metabolism and detoxification mainly occur in the liver, so the liver is the most easily affected organ by drugs. Hepatotoxicity is one of the items that must be tested for drug safety.
The evaluation of the effects of exogenous drugs on liver injury and the methods of liver toxicology can be divided into two parts, including in vivo and in vitro. The above two methods have their own characteristics.
- In vivo. The living animal can be used for long-term dynamic observation and the effects of chronic toxicity on the organism can also be observed in the same animal. The results of in vivo can be extrapolated to people.
- In vitro. Firstly, it is easy to control environmental factors in in vitro trials. Secondly，the effects of interacting systems such as immune and neuroendocrine systems can be excluded. Thirdly, under the condition of small experimental error, in vitro trials are fast and high cost-effective. The greatest strength of in vitro is the ability to experiment with liver cells or tissue, therefore the mechanism of liver injury caused by exogenous compounds can be studied at different levels of tissues, cells and molecules.
Creative Biolabs's hepatotoxicity detection service is designed to provide customers with the best toxicity assay techniques. Through a variety of application platform testing, Creative Biolabs can meet the needs of different types of liver toxicity assay, helping customers choose and optimize new drugs in the early stages of development. With a professional technical team and advanced experimental equipment, Creative Biolabs provides scientific research workers and institutions with impeccable technical cooperation and guidance of transformation service.
We provide but are not limited to:
- In vivo. 1. Assay for serum enzyme of hepatocyte injury. (1) Alanine aminotransferase (ALT) levels; (2) Aspartate transaminase (AST) levels; (3) Alkaline phosphatase (ALP) levels; (4) Lactic dehydrogenase (LDH) levels; (5) Glutamyl transferase (GGT) levels. 2. Liver function tests. (1) Excretory function of the liver; (2) Secretory function of the liver. 3. Hepatic fibrosis assay.
- In vitro. 1. Liver Perfusion. In vitro liver perfusion can preserve the structural and functional integrity of hepatocytes to a certain extent, maintaining the cell membrane barrier and the supply of body fluid. In this way, it is possible to dynamically study the metabolic changes of drugs in the liver and their effects on liver function under the condition of artificially controlling dose and excluding the overall effect. 2. Primary culture of liver cells. Liver cells from primary culture can be used to carry out many toxicological studies, such as the hepatotoxicity of drugs. It is generally believed that the screening and identification of hepatotoxicity of the drugs by hepatocytes from primary culture are reliable and consistent with in vivo experiments.
- Cytochrome P450 enzyme system (CYP450). Induction or inhibition of CYP450 by drugs is an important cause of drug interaction in clinic. In recent years, the research on CYP450 and its related enzyme system is very deep and extensive in biochemistry and toxicology. The most abundant organ of CYP450 in mammals is the liver. Therefore, the induction or inhibition of CYP450 by drugs and their subsequent effects are also an important part of liver toxicology.
Featured technical service：
- Based on the high-content screening (HCS) platform, Creative Biolabs builds a variety of cell state analysis model that can analyze cell changes easily, quickly and multi-index, carrying out large-scale screening of small molecules, nucleic acid drugs.
The Creative Biolabs HCS platform is equipped with a variety of advanced equipment and data processing system to achieve the full automation of high-content screening, providing analysis and screening of cell health and toxicity.
Advantages of our hepatotoxicity detection services:
- Rich experience in sample handling.
- Perfect experimental skills.
- High cost performance.
- Impeccable biotechnology products & services.
References
- Mcdonnell M E, et al. Drug-Related Hepatotoxicity. New England Journal of Medicine, 2006, 354(20):2191-2193.
- Jaeschke H, et al. Mechanisms of Hepatotoxicity. Toxicological sciences, 2002, 65(2):166-176.
- Shuai Z L, et al. Review of high content screening applications in toxicology. Archives of Toxicology, 2019, 93(12): 3387-3396.
*For Research Use Only. Not for use in diagnostic procedures.
