Nuclear Magnetic Resonance Spectroscopy

NMR spectroscopy is suitable for all stages of pre-clinical research and development of new nucleic acid drugs, and can detect the nature of organic compounds and the interaction of their surrounding chemical environment.

Nuclear magnetic resonance spectroscopy (NMR) can be applied to the following research:

1. Spatial structure analysis of drug target molecules such as proteins and nucleic acid molecules;
2. Structure analysis of small molecule lead compounds and natural products;
3. Research on the molecular mechanism of protein-ligand interaction;
4. High-throughput screening of small molecules;
5. Research on drug structure-activity relationship;
6. Toxicology and safety evaluation of new drugs, etc.

Creative Biolabs is a professional biotechnology service platform that provides pre-clinical evaluation technology. Operating in accordance with global regulations and fully accredited by AAALAC, Creative Biolabs ensure the overall health and safety of the laboratory and reduce risks to customers. Our researchers are all from certified organizations and institutions, and have undergone rigorous training to ensure that the quality of nuclear magnetic resonance spectroscopy structure determination services meets national standards.

We provide but are not limited to:

• Determine the spatial structure of drug target molecules
• Analyze the structure of small molecule lead compounds and natural products
• Study the molecular mechanism of protein-ligand interactions
• High-throughput screening of small molecules
• Identify the structure-activity relationship of a drug
• Toxicology and safety evaluation of new drugs

Classification of NMR spectrometers:

Choose the NMR analyzer suitable for samples:

	Continuous wave NMR spectrometer	Pulse Fourier Transform NMR Spectrometer
Principle	The RF field is continuously applied to the sample, a single frequency is emitted, and a resonance spectrum line is obtained	With a constant magnetic field, a strong and short radio frequency pulse is used to irradiate the sample, and the induced current signal is Fourier transformed to obtain a general nuclear magnetic resonance spectrum.
Features	Long time, usually full scan time is 200-300 seconds Low sensitivity The amount of sample required is large For some samples that are difficult to obtain, NMR analysis cannot be performed	High sensitivity (100 times) Measurement speed is fast. Generally, 1 h NMR measurement is accumulated 10-20 times, and it takes about 60 s Small sample amount

General procedure of NMR spectroscopy analysis:

• Preparation of NMR tube: Choose a NMR tube of appropriate specifications, and ensure that it is cleaned and dried.
• Preparation of sample solution: Choose a suitable solvent and control the concentration of the sample solution.
• Shimming treatment before testing: Load the NMR tube into the instrument, rotate it, and perform shimming.
• Sample scan: According to the molecular weight of the sample, select the appropriate scan times.
• Result analysis: save the data and use special software to analyze the spectrum.

Advantages of NMR spectroscopy analysis services:

• Rapidity.
• High sensitivity.
• High sample throughput.
• Integrity and system: NMR single measurement can simultaneously detect and analyze primary (such as carbohydrates, amino acids) and secondary metabolites.
• Strong structure derivation and identification capabilities: it can clarify the structure of unknown chemical components.
• Accurate quantitative analysis can be performed without preparing a standard curve: the peak area of the NMR signal is directly proportional to the number of protons that cause the signal.
• Sample preparation is simple: the medicinal material samples can be extracted directly with deuterated reagents, and the analysis is fast.
• Universality: As long as the chemical composition contains protons, it can be detected and analyzed.

References

1. Holmes E, Tang H R, Wang Y L, et al. The assessment of plant metabolite profiles by NMR-based methodologies. Planta Med, 2002, 72: 771-785.
2. Frank V D K , Maltese F , Choi Y H, et al. Quality Control of Herbal Material and Phytopharmaceuticals with MS and NMR Based Metabolic Fingerprinting. Planta Med, 2009, 75 (07):763-775.
3. Kim H K, Choi Y H, Verpoorte R. NMR-based metabolomic analysis of plant. Nature protocols, 2010, 5 (3): 536-549.
4. Ujjayini Ghosh, David P. Weliky. 2H nuclear magnetic resonance spectroscopy supports larger amplitude fast motion and interference with lipid chain ordering for membrane that contains β sheet human immunodeficiency virus gp41 fusion peptide or helical hairpin influenza virus hemagglutinin fusion peptide at fusogenic pH. BBA-Biomembranes, 2020.

*For Research Use Only. Not for use in diagnostic procedures.

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