RNA Probe Synthesis Services

The RNA probe refers to a single-stranded cDNA or cRNA molecule with a label that can complementarily bind to the corresponding nucleotide sequence in the tissue. As a kind of promising nucleic acid probes, RNA probes are mainly used for research purposes. RNA probe detection is fast, simple and sensitive.

As an important nucleic acid probe, RNA probe has been widely used in biomedical field: molecular biology research, research on genetic disease genes, genetic diagnosis, molecular in situ hybridization, virus identification test, diagnosis of infectious diseases and tumor early screening research.

Creative Biolabs is a professional bio-technology services platform providing outsourced oligonucleotide synthesis services. We have professional researchers and strict quality control, can provide there types of the following RNA probe synthesis.

The types of RNA probes:

	cRNA probe	Single-stranded cDNA probe	Oligonucleotide probe
Features	It is obtained by in vitro transcription using cDNA as a template.	No introns and other highly repetitive sequences in cDNA.	It uses nucleotides as raw materials and is synthesized by a DNA synthesizer to avoid the adverse effects of highly repetitive sequences in eukaryotic cells.
Advantages	The hybrid formed between cRNA and RNA has strong stability The hybrid formed between cRNA-RNA is not affected by RNase. High level of hybridization saturation of cRNA probe.	Overcoming the shortcomings of double-stranded cDNA probe renaturation between the two strands in the hybridization reaction. Improved sensitivity of hybridization reaction.	Most oligonucleotides have short sequences, do not require purification, and have excellent tissue penetration. Strong specificity.

Synthesis method of RNA probe:

• Enzymatic reaction method
• End labeling method: It is a labeling method to introduce the label into the 5 or 3 ends of linear DNA or RNA. The end labeling is suitable for the labeling of synthetic oligonucleotide probes. The probes prepared with the end labeling are generally carried There are fewer marker molecules.
• In vitro transcription: It is a method of preparing single-stranded RNA probes with the same sequence as the fragment.



In vitro transcription synthesis flow chart

• Chemical reaction method

Chemical synthesis is a process of synthesizing RNA probe by using compounds as templates, PCR and other biological means combined with RNA probe transcription in vitro.

References

1. Golab Karolina, Krzystyniak Adam, et al. Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization Biology&Molecular Biology. 2020.
2. Thomas J. X. Li, et al. On an enhancement of RNA probing data using information theory. Algorithms for Molecular Biology. 2020, 15(1): 167-212.
3. Dresselhaus Thomas, Bleckmann. Andrea Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization. Methods in molecular biology. 2020, 2166: 3-21.

*For Research Use Only. Not for use in diagnostic procedures.

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